Vitae is of cosmic origin and causes the living host to burst with energy. The catalyst is produced somewhere in the brain. Although many of its properties are unknown, one of them is the ability to extend life, potentially for centuries, if consumed in large amounts. In order to procure such amounts, it must be acquired from humans. Alexander found out through torturing dogs that although animals also secrete Vitae, they do not produce it in large enough doses to be of any use (See Canis Lupus Familiaris Note).
Obtaining Vitae is a methodical and painstaking task, as torture must be inflicted in the right fashion for maximum Vitae extraction. When pain or fear is instilled in a victim, the body fills the bloodstream with Vitae, and this can result in fits of violence and brute strength if the victim is not properly restrained and terrified. After the victim is completely broken, they will no longer produce enough Vitae, as their mind has already adjusted to the torture. Thus, Alexander used an amnesia drink to make them forget about the torture, so more Vitae could be harvested.
Alexander made several attempts to create an artificial form of Vitae in the Laboratory. However, each one ended in failure. The otherworldly being was unable to grasp the mysterious inner workings of life and their relation to the mystic energy. His last experiment's chemical solution was rendered highly acidic and useless for the intended purposes as it ironically dissolved organic matter instead.
After the events of Amnesia: The Dark Descent, 60 years later, Oswald Mandus discovers the effects of Vitae after witnessing a worker's severed head come to life after being drenched in the Brennenburg compound and starts using Vitae in his experiments. Calling it Brennenburg Infusion Vitae, he uses it in conjunction with another substance, the Orgone Monad Disperal fluid to create his Manpigs. It is unknown whether Mandus discovered Vitae on his own or simply continued and improved on the work of Alexander.
- The Vitae from Amnesia: The Dark Descent shares a slight resemblance with Azoth from Haunting Ground. Both Vitae and Azoth are extracted from tortured humans, and occasionally animals, and can extend one's life for centuries. The tortured victims will most likely die from the extraction.
- "Vitae" translates to "Life" in Latin.
- Ethanol was commonly called aqua vitae ('life water') by alchemists throughout the Middle Ages and Renaissance.
- Many of the effects of vitae (such as increased strength in stressful situations) are similar to the effects caused by adrenaline.